Forward light scattering analysis of particles and cells

Information about forward light scattering properties of particles and biological cells is important for the understanding of imaging in optical microscopy, optical trapping and high throughput measurement techniques like flow cytometry. Digital holography provides the storage and the numerical reconstruction of wave fields. If an off-axis setup is used for hologram recording, the wave field behind particle-like scatterers can be calculated in amplitude and phase from a single digital hologram. Data from digital holographic microscopy measurements on microspheres in different immersion media and from different cell types are analysed and compared to results from simulations.