Concept and implementation of a compact multi-channel fluorescence-microscope unit

Eike Slogsnat1, L. Lehmann2, Peer Fischer2, Karl-Heinz Brenner1
  1. 1Chair of Optoelectronics, Institute of Computer Engineering, Heidelberg University
  2. 2Chair of Circuit Design, Institute of Computer Engineering, Heidelberg University

eike [dot] slogsnat [at] ziti [dot] uni-heidelberg [dot] de

Due to the large number of single experiments in systems biology there is a demand for accelerating image acquisition. We present the concept and the implementation of a compact multi-spectral microscope unit, which is able to acquire images of four fluorophores in parallel. The unit consists of four excitation channels and four emission channels realized by dichroic filters. The imaging is performed by one standard objective lens for all channels and one tubus lens and one camera for each emission channel. By combining multiple units, a parallelization in the spatial domain can be achieved.

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@inproceedings{slogsnat2012p2,
  author     = {Slogsnat, Eike and Lehmann, L. and Fischer, Peer and Brenner, Karl-Heinz},
  title      = {{Concept and implementation of a compact multi-channel fluorescence-microscope unit}},
  booktitle  = {DGaO-Proceedings 2012},
  series     = {DGaO-Proceedings},
  eventtitle = {113. Jahrestagung der DGaO},
  eventdate  = {2012-05-30/2012-06-02},
  venue      = {Eindhoven},
  publisher  = {Deutsche Gesellschaft für angewandte Optik e.V.},
  year       = {2012},
  pages      = {P2},
  langid     = {english},
  url        = {https://dgao-proceedings.de/paper/113-p2}
}
113. Annual Conference of the DGaO · Eindhoven · 2012