Raman spectroscopy for reproducible bacterial and yeast biofilm identification

We report on Raman spectroscopy measurements for the selected bacterial and yeast strains (biofilm positive and biofilm negative) on colonies grown directly on the Petri dishes. Chemometric principal component analysis of these spectra sets generated clusters of data points, from which the reproducibility of the measurement could be analysed. Consequently, these resulted in clusters coinciding well with the biofilm positive and biofilm negative strains measurement of a particular sample dish, suggesting good reproducibility of our measurement procedure, even when the samples were prepared and measured days up to months apart. This suggests the potential of Raman spectroscopy in routine clinical diagnostic. Acknowledgements This work received support from the Czech Science Foundation (GACR P205/11/1687) and the Ministry of Health, Ministry of Education, Youth and Sports of the Czech Republic (LO1212) together with the European Commission (ALISI No. CZ.1.05/2.1.00/01.0017).